PCR Equipment and Supplies
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Applied Biosystems™ Protein Thermal Shift™ Dye Kit
This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit
The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript III |
| Size (Final Product) | Up to 4.5 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | Cells |
| For Use With (Application) | Real Time PCR (qPCR) |
Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit
The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi U DNA Polymerase |
| GC-Rich PCR Performance | High |
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 10 kb |
| Final Product Type | PCR Amplified cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription, Preamplification |
| Starting Material | Cells or RNA |
| For Use With (Application) | Next-Generation Sequencing (NGS),Real Time PCR (qPCR) |
Applied Biosystems™ ProFlex™ PCR System + Extended Warranty + Plastics Packages, 2 x 96-well
Applied Biosystems ProFlex PCR systems provide the reliability and performance you’ve come to expect from Applied Biosystems instruments.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Block Configurations | 2 x 96-well Isothermal Blocks |
|---|---|
| Display Type | 8.4 in. Color TFT LCD Touchscreen |
| Product Type | Thermal Cycler |
| Capacity | 192-well, 0.2 mL Tubes |
| Reaction Speed | Fast, Standard |
| User Interface | USB, WiFi, Cloud, Ethernet |
| Voltage | 100/240 V |
| Quantity | 1 Package |
| Dimensions | 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm) |
| Max. Ramp Rate | 3°C/sec. (Block), 1.6°C/sec. (Sample) |
| High-throughput Compatibility | Manual Protocols |
| Weight | 41 lb. (18.8 kg) |
| Thermal Range | 0°C to 100°C |
Applied Biosystems™ ProFlex™ PCR System + Extended Warranty + Plastics Packages, 2 x 384-well
Applied Biosystems ProFlex PCR systems provide the reliability and performance you’ve come to expect from Applied Biosystems instruments.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Block Configurations | 2 x 384-well Isothermal Blocks |
|---|---|
| Display Type | 8.4 in. Color TFT LCD Touchscreen |
| Product Type | Thermal Cycler |
| Capacity | 768 x 20 μL Tubes, 2 x 384-well Plate |
| Reaction Speed | Fast, Standard |
| User Interface | USB, WiFi, Cloud, Ethernet |
| Voltage | 100/240 V |
| Quantity | 1 Package |
| Dimensions | 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm) |
| Max. Ramp Rate | 3°C/sec. (Block), 1.6°C/sec. (Sample) |
| High-throughput Compatibility | Manual Protocols |
| Thermal Range | 0°C to 100°C |
Invitrogen™ AccuPrime™ Pfx DNA Polymerase
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | AccuPrime Pfx DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 26X |
| Size (Final Product) | 12 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
| Detection Method | Primer-probe |
Invitrogen™ Oligo(dT)12-18 Primer
Oligo(dT)12-18 Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 2 X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq DNA Polymerase |
| Format | Tube |
| Product Type | Fast Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Product Line | AmpErase, TaqMan |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Polymerase | AmpliTaq Gold DNA Polymerase |
| GC-Rich PCR Performance | High |
| Product Type | Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Invitrogen™ SuperScript™ VILO™ Master Mix
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| Concentration | 5X |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
| Format | Master Mix |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Master Mix |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | Platinum Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Starting Material | DNA |
| For Use With (Application) | Hot-start PCR |
| Exonuclease Activity | 5' - 3' |
Applied Biosystems™ QuantStudio™ 5 Dx Real-Time PCR System, laptop
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems QuantStudio 5 Dx Real-Time PCR System is an in vitro diagnostic (IVD) quantitative PCR system that delivers proven performance and support to help meet the needs of clinical/hospital laboratories and test developers, in a compact footprint and cost-effective package.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ QuantStudio™ 5 Dx Real-Time PCR System, desktop
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems QuantStudio 5 Dx Real-Time PCR System is an in vitro diagnostic (IVD) quantitative PCR system that delivers proven performance and support to help meet the needs of clinical/hospital laboratories and test developers, in a compact footprint and cost-effective package.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Platinum™ Taq DNA Polymerase, High Fidelity
Platinum Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum Taq DNA Polymerase High Fidelity |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 6X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Array Human Endogenous Control
TaqMan™ Express Endogenous Control Plates use TaqMan™ probe-based chemistry and are designed for use on the suite of Applied Biosystems™ Real-Time PCR Systems.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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